Expression of Winged Bean Basic Agglutinin in Spodoptera frugiperda insect cell expression system

Srinivas, VR and Bachhawat-Sikder, Kiran and Habib, Saman and Hasnain, Seyed E and Surolia, Avadhesha (2001) Expression of Winged Bean Basic Agglutinin in Spodoptera frugiperda insect cell expression system. In: Bioscience Reports, 21 (3). pp. 361-367.

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Abstract

In this paper we report the successful expression of the winged bean basic agglutinin (WBA I) in insect cells infected with a recombinant baculovirus carrying the WBA I gene and its characterization in terms of its carbohydrate binding properties. The expressed protein appears to have a lower molecular weight than the native counterpart which is consistent with the lack of glycosylation of the former. Moreover, the expressed protein maintains its dimeric nature. Hence, a role for glycosylation in modulation of dimerization of WBA I is ruled out unlike Erythrina corallodendron (EcorL). Despite this the protein is active, with its sugar specificity unaltered.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Portland Press.
Keywords:	Legume lectins;winged bean basic lectin;Baculovirus-insect cell expression system.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	07 Aug 2008
Last Modified:	19 Sep 2010 04:48
URI:	http://eprints.iisc.ernet.in/id/eprint/15503

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