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An internally quenched fluorescent substrate for collagenase

Saikumari, YK and Balaram, P (2008) An internally quenched fluorescent substrate for collagenase. In: Peptide Science, 90 (2). pp. 131-137.

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Abstract

A synthetic collagenase substrate containing the internal peptide sequence—Gly-Gly-Pro-Leu-Gly-Pro-Pro-Gly- Pro—has been synthesized, with an N-terminus 4-((4-(dimethylamino)phenyl)azo)-benzoyl (DABCYL) group and C-terminus 5-[2-(acetamido)ethylamino] naphthalene-1-sulfonic acid (AEDANS) moiety resulting in internal quenching of AEDANS fluorescence. Peptide bond hydrolysis results in a large increase in fluorescence at 490 nm upon excitation at 336 nm. The substrate is cleaved exclusively by Clostridium histolyticum collagenase and is completely resistant to attack by proteases like thermolysin, proteinase K, and trypsin. $K_m$ and $V_{max}$ values for substrate hydrolysis by collagenase have been determined, establishing the peptide as one of the best binding substrates for the enzyme. MALDI mass spectrometry using a derivative of the substrate establishes that the sites of cleavage lie within the collagen like domain. The CD spe ctrum of an analog peptide lacking the donor and acceptor groups reveals spectral features that are reminiscent of weak polyproline structures.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Wiley Periodicals.
Keywords: Clostridium histolyticum;collagenase;internally quenched fluorescent substrate;MALDI mass spectrometry;circular dichroism.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 18 Aug 2008
Last Modified: 19 Sep 2010 04:49
URI: http://eprints.iisc.ernet.in/id/eprint/15555

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