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LysR type Transcriptional Regulator, Hrg Counteracts Phagocyte Oxidative Burst and Imparts Survival Advantage to Salmonella enterica serovar Typhimurium

Lahiri, Amit and Das, Priyanka and Chakravortty, Dipshikha (2008) LysR type Transcriptional Regulator, Hrg Counteracts Phagocyte Oxidative Burst and Imparts Survival Advantage to Salmonella enterica serovar Typhimurium. In: Microbiology, 154 (9). pp. 2837-2846. (In Press)

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Abstract

LysR type transcriptional regulators are one of the key players that help bacteria adapt to different environments. We have christened STM0952, a putative LysR type transcriptional regulator in Salmonella enterica serovar Typhimurium as hydrogen peroxide resistance gene (hrg). By generating a knock out of the hrg gene, we demonstrate that the hrg mutant serovar Typhimurium is sensitive to oxidative products of the respiratory burst, specifically to hydrogen peroxide. The hrg mutant is profoundly attenuated in murine model of infection and shows decreased intracellular proliferation in macrophages. It was also found to induce increased amount of reactive oxygen species and co-localization with gp91phox in the macrophage cell line, when compared to the wild type. An overproducing strain of this gene showed a survival advantage over the wild type Salmonella under hydrogen peroxide induced stress condition. Microarray analysis suggested the presence of an Hrg regulon, which is required for resistance to the toxic oxidative products of the reticulo-endothelial system.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Society for General Microbiology.
Keywords: Salmonella enterica serovar Typhimurium;hrg;oxidative stress;gp91phox.
Department/Centre: Division of Biological Sciences
Date Deposited: 19 Aug 2008
Last Modified: 27 Aug 2008 13:44
URI: http://eprints.iisc.ernet.in/id/eprint/15653

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