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Role of actin cytoskeleton in LPS-induced $NF-\kappa B$ activation and nitric oxide production in murine macrophages

Eswarappa, Sandeepa M and Pareek, Vidhi and Chakravortty, Dipshikha (2008) Role of actin cytoskeleton in LPS-induced $NF-\kappa B$ activation and nitric oxide production in murine macrophages. In: Innate Immunity, 14 (5). pp. 309-318. (In Press)

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Abstract

Lipopolysaccharide (LPS) is a major cell wall component of Gram-negative bacteria and is known to cause actin cytoskeleton reorganization in a variety of cells including macrophages. Actin cytoskeleton dynamics influence many cell signaling pathways including the $NF-\kappa B$ pathway. LPS is also known to induce the expression of many pro - inflammatory genes via the $NF-\kappa B$ pathway. Here, we have investigated the role of actin cytoskeleton in LPS-induced $NF-\kappa B$ activation and signaling leading to the expression of iNOS and nitric oxide production. Using murine macrophages, we show that disruption of actin cytoskeleton by either cytochalasin D (CytD) or latrunculin B (LanB) does not affect LPS-induced $NF-\kappa B$ activation and the expression of iNOS, a $NF-\kappa B$ target gene. However, disruption of actin cytoskeleton caused significant reduction in LPS-induced nitric oxide production indicating a role of actin cytoskeleton in the post-translational regulation of iNOS.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to SAGE publications.
Keywords: Actin;cytochalasin D;iNOS;latrunculin B;lipopolysaccharide;NF-\{kappa}B
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 16 Oct 2008 07:29
Last Modified: 16 Oct 2008 07:29
URI: http://eprints.iisc.ernet.in/id/eprint/15905

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