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In vitro polymerization of Mycobacterium leprae FtsZ OR Mycobacterium tuberculosis FtsZ is revived or abolished, respectively, by reciprocal mutation of a single residue

Gupta, Prabuddha and Srinivasan, Ramanujam and Rajeswari, Haryadi and Indi, Shantinath and Ajitkumar, Parthasarathi (2008) In vitro polymerization of Mycobacterium leprae FtsZ OR Mycobacterium tuberculosis FtsZ is revived or abolished, respectively, by reciprocal mutation of a single residue. In: Biochemical and Biophysical Research Communications, 368 (2). pp. 445-452.

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Abstract

A single residue that dramatically influences polymerization of principal cell division protein FtsZ of Mycobacterium leprae (MlFtsZ) and Mycobacterium tuberculosis (MtFtsZ) has been identified. Soluble, recombinant MlFtsZ did not show polymerization in vitro, in contrast to MtFtsZ, which polymerised. Mutation of the lone non-conserved residue T172 in the N-terminal domain of MlFtsZ to A172, as it exists in MtFtsZ, showed dramatic polymerization of MlFtsZ–T172A in vitro. Reciprocal mutation of A172 in MtFtsZ to T172, as it exists in MlFtsZ, abolished polymerization of MtFtsZ–A172T in vitro. While T172A mutation enhanced weak GTPase activity of MlFtsZ, reciprocal A172T mutation marginally reduced GTPase activity of MtFtsZ in vitro. These observations demonstrate that the residue at position 172 plays critical role in the polymerization of MlFtsZ and MtFtsZ. A possible evolutionary correlation between the presence of polymerization-adversive or polymerization-favouring residue at position 172 in FtsZ and generation time of the respective bacterium are discussed.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: FtsZ;Mycobacteria;Bacterial cell division;Polymerization;GTPase;GTP binding.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 25 Sep 2008 08:59
Last Modified: 19 Sep 2010 04:50
URI: http://eprints.iisc.ernet.in/id/eprint/16005

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