Basak, Shashwati and Nagaraja, V (2001) A versatile in vivo footprinting technique using 1,10-phenanthroline-copper complex to study important cellular processes. In: Nucleic Acids Research, 29 (21). e105-1-e105-6.
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A number of reagents have been used to define the sequence-specific protein-DNA contacts by footprinting analysis. We report a new in vivo technique using the complex of 1,10-phenanthroline and copper $[(OP_2)Cu]$ as a probe to study various intracellular DNA-protein interactions in wholecells. The versatility of the protocol is demonstrated by applying the technique to address various processes. The protocol is applied to (i) detect structural alterations in DNA as a result of single base substitution, (ii) footprint site-specific DNA-binding proteins, (iii) analyze promoter occupancy by RNA polymerase and (iv) analyze molecular interactions during transcription initiation. The results demonstrate that in vivo $(OP)_2Cu$ probing is a useful tool in studying important cellular processes involving DNA-protein interactions and has potential applications in post-genomic research.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Oxford University Press.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||13 Oct 2008 07:22|
|Last Modified:||19 Sep 2010 04:50|
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