Identification of C-terminal neighbours of amino acid residues without an aliphatic $^{13}C^\gamma$ as an aid to NMR assignments in proteins

Barnwal, Ravi Pratap and Rout, Ashok K and Atreya, Hanudatta S and Chary, Kandala VR (2008) Identification of C-terminal neighbours of amino acid residues without an aliphatic $^{13}C^\gamma$ as an aid to NMR assignments in proteins. In: Journal of Biomolecular NMR, 41 (4). pp. 191-197.

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Abstract

We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Isotope labeling;NMR;Automated assignments;Sequence specific resonance assignments.
Department/Centre:	Division of Chemical Sciences > NMR Research Centre (Formerly SIF)
Date Deposited:	14 Oct 2008 07:25
Last Modified:	19 Sep 2010 04:50
URI:	http://eprints.iisc.ernet.in/id/eprint/16054

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