Ramani, B and Kondaiah, P (1998) Recombinant expression of human transforming growth factor-beta isoforms in Chinese hamster ovary cells. In: Journal of Biosciences, 23 (5). pp. 577-583.
Transforming growth factor-beta s (TGF-beta s) are multi functional growth modulators implicated in several physiological processes which include embryogenesis, inflammation, immune-suppression, wound healing, carcinogenesis and cellular differentiation. For clinical use, recombinant expression of TGF-beta s is the only practical source because of very low yields from natural sources. Here, we report the recombinant expression of human TGF-beta 1 and TGF-beta 2 in a mammalian expression system using a high expression eukaryotic vector driven by a cytomegalovirus promoter. Expression levels are as high as 0.97 mu g/ml of TGF-beta 1 and 0.24 mu g/ml of TGF-beta 2 in conditioned media, sufficient for purification without the need for amplification of the gene using methotrexate.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Indian Academy of Sciences.|
|Keywords:||Recombinant DNA;gene expression;growth factors;TGF-beta;CHO cells|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)|
|Date Deposited:||04 Oct 2004|
|Last Modified:||19 Sep 2010 04:15|
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