Barnwal, Ravi Pratap and Rout, Ashok K and Chary, Kandala VR and Atreya, Hanudatta S (2007) Rapid measurement of $^3J(H^N-H^\alpha)$ and $^3J(N-H^\beta)$ coupling constants in polypeptides. In: Journal of Biomolecular NMR, 39 (4). pp. 259-263.
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We present two NMR experiments, (3,2)D HNHA and (3,2)D HNHB, for rapid and accurate measurement of $(^3J(H^N-H^\alpha)$ and $^3J(N-H^\beta)$ coupling constants in polypeptides based on the principle of G-matrix Fourier transform NMR spectroscopy and quantitative J-correlation. These experiments, which facilitate fast acquisition of three-dimensional data with high spectral/digital resolution and chemical shift dispersion, will provide renewed opportunities to utilize them for sequence specific resonance assignments, estimation/characterization of secondary structure with/without prior knowledge of resonance assignments, stereospecific assignment of prochiral groups and 3D structure determination, refinement and validation. Taken together, these experiments have a wide range of applications from structural genomics projects to studying structure and folding in polypeptides.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Springer.|
|Keywords:||Coupling constants;GFT NMR;Protein Structures;Protein folding;Resonance assignment|
|Department/Centre:||Division of Chemical Sciences > NMR Research Centre (Formerly SIF)|
|Date Deposited:||18 Dec 2008 07:18|
|Last Modified:||19 Sep 2010 04:52|
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