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Agrobacterium tumefaciens-mediated transformation of eggplant ( Solanum melongenaL.) using root explants

Franklin, G and Sita, Lakshmi G (2003) Agrobacterium tumefaciens-mediated transformation of eggplant ( Solanum melongenaL.) using root explants. In: Plant Cell Reports, 21 (6). pp. 549-554.

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Abstract

An efficient variety-independent method for producing transgenic eggplant (Solanum melongena L.) via Agrobacterium tumefaciens-mediated genetic transformation was developed. Root explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pBAL2 carrying the reporter gene \beta-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calli were induced in media containing 0.1 mg$ l-^{1}$ thidiazuron (TDZ), 3.0 mg $l-^{1} N^{6}$-benzylaminopurine, 100 mg$ l-^{1}$ kanamycin and 500 mg l? cefotaxime. The putative transgenic shoot buds elongated on basal selection medium and rooted efficiently on Soilrite irrigated with water containing 100 mg$ l-^{1} $kanamycin sulphate. Transgenic plants were raised in pots and seeds subsequently collected from mature fruits. Histochemical GUS assay and polymerase vchain reaction analysis of field-established transgenic plants and their offsprings confirmed the presence of the GUS and NPTII genes, respectively. Integration of T-DNA into the genome of putative transgenics was further confirmed by Southern blot analysis. Progeny analysis of these plants showed a pattern of classical Mendelian inheritance for both the NPTII and GUS genes.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer-Verlag
Keywords: Eggplant;Agrobacterium;Transformation;Glucuronidase
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 03 Dec 2008 07:43
Last Modified: 08 Jul 2011 09:33
URI: http://eprints.iisc.ernet.in/id/eprint/16573

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