Mathew, Renjith and Ojha, AK and Karande, AA and Chatterji, Dipankar (2004) Deletion of the rel gene in Mycobacterium smegmatis reduces its stationary phase survival without altering the cell-surface associated properties. In: Current Science, 86 (1). pp. 149-153.
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Dormant or latent physiology of the mycobacterial species is a subject of current investigation in order to understand the long-term persistence of these organisms inside the host. It is argued that the carbon-starved mycobacteria may serve as a good model for the dormant bacilli. The relA/spoT gene is upregulated during carbon starvation in Mycobacterium tuberculosis and the deletion of the gene resulted in reduction of long-term persistence in M. tuberculosis. Overexpression of the gene in M. smegmatis changes the colony morphology of the bacteria. Here we show that knock-out of the relA/spoT gene compromises stationary-phase survival of M. smegmatis. However, the DeltarelA/spoT bacteria show similar profile of glycopeptidolipids as the wild-type bacteria under carbon starvation. We have seen here that M. smegmatis, a nonpathogenic species, upon carbon starvation exhibits reduced association with murine macrophage cell line RAW 264.7 in comparison to M. smegmatis grown in carbon-enriched medium. But the clearance of the a bacteria from macrophages takes place in the same window of time in both conditions.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Biochemistry
|Date Deposited:||18 Dec 2008 11:04|
|Last Modified:||19 Sep 2010 04:53|
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