FM1-43 measurements of local exocytotic events in rat melanotrophs

Sikdar, SK and Kreft, M and Pangrsic, T and Gril, S and Zorec, R (2005) FM1-43 measurements of local exocytotic events in rat melanotrophs. In: FEBS Letters, 579 (29). pp. 6575-6580.

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Abstract

We have explored the existence of fusion- and secretion-competent sites on the plasma membrane of peptide secreting rat pituitary melanotrophs at rest, and following stimulation with glutamate. We monitored changes in fluorescence of FM1-43, a styryl dye which labels plasma membrane. The results show spontaneous local increases in FM1-43 reporting changes in membrane surface area due to cumulative exocytosis. Addition of glutamate, further increased the occurrence of these events. Statistical analysis of local FM1-43 fluorescence changes suggests that this is due to the recruitment of inactive exocytotic domains and due to the stimulation of already active exocytotic domains.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Neuroendocrine cell;Exocytosis;FM1-43;Hormone secretion;Exocytotic domain.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	21 Jul 2009 14:55
Last Modified:	09 Jan 2013 06:40
URI:	http://eprints.iisc.ernet.in/id/eprint/17041

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