Ghosh, Debalina and Faure, Nicolas and Kundu, Sharmistha and Rondelez, Francis (2003) Hybridization and immobilization of long ds-DNAs on polystyrene microspheres. In: Langmuir, 19 (14). pp. 5830-5837.
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Here, we develop a system where long double-stranded DNAs (ds-DNAs) are immobilized on the surface of a polystyrene (PS) microsphere. A simple synthetic strategy is adopted in order to achieve this goal in which a single DNA chain is anchored by one of its extremities to a latex (PS) microsphere. We chose hybridization as a unique method to attach long ds-DNA chains in solution with oligonucleotides grafted on modified aminated polystyrene microspheres. The DNAs chosen were of various sizes and sources: T7A1 DNA (4.48 kilobase pair (kbp)), a plasmid DNA; and DeltaDIIIT7 DNA (39.34 kbp), a mutant of bacteriophage T7 DNA. The temperature dependence of the kinetics of hybridization of T7A1 DNA (4.48 kbp) in solution with an appropriate oligonucleotide (20-mer sequence) grafted on modified aminated polystyrene microspheres yielded a value of activation energy of similar to5.3 kcal/mol, consistent with a non-diffusion-controlled mechanism.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Chemical Society|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||12 Aug 2009 09:34|
|Last Modified:||19 Sep 2010 04:55|
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