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Hybridization and immobilization of long ds-DNAs on polystyrene microspheres

Ghosh, Debalina and Faure, Nicolas and Kundu, Sharmistha and Rondelez, Francis (2003) Hybridization and immobilization of long ds-DNAs on polystyrene microspheres. In: Langmuir, 19 (14). pp. 5830-5837.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/la0341963

Abstract

Here, we develop a system where long double-stranded DNAs (ds-DNAs) are immobilized on the surface of a polystyrene (PS) microsphere. A simple synthetic strategy is adopted in order to achieve this goal in which a single DNA chain is anchored by one of its extremities to a latex (PS) microsphere. We chose hybridization as a unique method to attach long ds-DNA chains in solution with oligonucleotides grafted on modified aminated polystyrene microspheres. The DNAs chosen were of various sizes and sources: T7A1 DNA (4.48 kilobase pair (kbp)), a plasmid DNA; and DeltaDIIIT7 DNA (39.34 kbp), a mutant of bacteriophage T7 DNA. The temperature dependence of the kinetics of hybridization of T7A1 DNA (4.48 kbp) in solution with an appropriate oligonucleotide (20-mer sequence) grafted on modified aminated polystyrene microspheres yielded a value of activation energy of similar to5.3 kcal/mol, consistent with a non-diffusion-controlled mechanism.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Chemical Society
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 12 Aug 2009 09:34
Last Modified: 19 Sep 2010 04:55
URI: http://eprints.iisc.ernet.in/id/eprint/17049

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