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G-protein activation modulates pseudo-periodic oscillation of Na channel

Majumdar, Sriparna and Sikdar, Sujit K (2006) G-protein activation modulates pseudo-periodic oscillation of Na channel. In: Biochemical And Biophysical Research Communications, 347 (2). pp. 444-451.

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Abstract

We have shown before that the duration and amplitude of both prolonged (1-160 s) and short (100-1000 ms) depolarizing prepulse altered all the steady-state and kinetic parameters of rNa(v)1.2a voltage-gated sodium channel in a pseudo-oscillatory fashion with variable time period and amplitude, often superimposed on a linear trend. In this study, we have examined the effect of G-protein activation on pseudo-oscillatory properties of the rNa(v)1.2a sodium channel alpha subunit, heterologously expressed in Chinese hamster ovary cells. G-protein modification caused insignificant changes in the slow pseudo-periodic oscillation of the activation properties of sodium channel; only the time period of the oscillation was altered from similar to 30 to 21 s. In contrast, G-protein activation abolished the faster component of pseudo-periodic oscillation in steady-state inactivation properties of sodium channel; the conditioning duration dependence of steady-state inactivation becomes monotonic in nature

Item Type: Journal Article
Additional Information: Copyright of this article belongs Elsavier.
Keywords: Voltage-gated sodium channel;G-protein;Periodic oscillation; Patch-clamp recording.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 24 Mar 2009 09:16
Last Modified: 19 Sep 2010 04:56
URI: http://eprints.iisc.ernet.in/id/eprint/17263

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