Chattopadhyay, A and Shaila, MS (2004) Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P. In: Virus Genes, 28 (2). pp. 169-178.
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The RNA dependant RNA polymerase of negative sense RNA viruses is composed of two subunits - the Large protein ( L) and the Phosphoprotein ( P). These two proteins have to form a complex in order to carry out genome transcription and replication. Employing the baculovirus expression system, we demonstrate here, the specific in vivo interaction between the L and P proteins of Rinderpest virus and also the stabilization of L protein when it is present as L+P complex. The regions on either protein involved in such interaction has been studied using the yeast two-hybrid system which indicates that the P binding region resides within the amino terminal 380 amino acid residues of L protein. The L binding region on P protein has been mapped to lie within 347 - 490 amino acids.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Kluwer Academic Publishers.|
|Keywords:||Interaction;Large protein;Phosphoprotein;Rinderpest virus.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||19 Dec 2008 15:11|
|Last Modified:||19 Sep 2010 04:56|
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