Banerjee, B and Balasubramanian, S and Ananthakrishna, G and Ramakrishnan, TV and Shivashankar, GV (2004) Tracking Operator State Fluctuations in Gene Expression in Single Cells. In: Biophysical Journal, 86 (5). pp. 3052-3059.
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We report the results of operator state fluctuations in gene expression for the entire bacterial growth cycle, using single-cell analysis and synthetic unregulated and negative-feedback transcription regulatory gene circuits. In the unregulated circuit, during the cell cycle, we observe a crossover from log-normal-to-normal distribution of expressed proteins and an unusual linear dependence of their standard deviation on the mean gene expression levels. With negative-feedback circuits we find the existence of bimodality as the cell cycle progresses. We suggest that such long-tail and bimodal distributions may be used as selection mechanisms in developmental switches and for assigning cell identity.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to The Biophysical Society.|
|Department/Centre:||Division of Chemical Sciences > Materials Research Centre
Division of Physical & Mathematical Sciences > Physics
|Date Deposited:||19 Dec 2008 18:48|
|Last Modified:||19 Sep 2010 04:56|
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