Bhat, VB and Madyastha, KM (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from Spirulina platensis: Protection against oxidative damage to DNA. In: Biochemical And Biophysical Research Communications, 285 (2). pp. 262-266.
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Peroxynitrite (ONOO-) is known to inactivate important cellular targets and also mediate oxidative damage in DNA, The present study has demonstrated that phycocyanin, a biliprotein from spirulina platensis and its chromophore, phycocyanobilin (PCB), efficiently scavenge ONOO-, a potent physiological inorganic toxin, Scavenging of ONOO- by phycocyanin and PCB was established by studying their interaction with ONOO- and quantified by using competition kinetics of pyrogallol red bleaching assay. The relative antioxidant ratio and IC50 value clearly indicate that phycocyanin is a more efficient ONOO- scavenger than PCB. The present study has also shown that PCB significantly inhibits the ONOO--mediated single-strand breaks in supercoiled plasmid DNA in a dose-dependent manner with an IC50 value of 2.9 +/- 0.6 muM. These results suggest that phycocyanin, has the ability to inhibit the ONOO--mediated deleterious biological effects and hence has the potential to be used as a therapeutic agent.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press.|
|Keywords:||Phycocyanin;phycocyanobilin;peroxynitrite scavenging;pyrogallol red;DNA damage;antioxidant|
|Department/Centre:||Division of Chemical Sciences > Organic Chemistry|
|Date Deposited:||10 Feb 2010 08:30|
|Last Modified:||19 Sep 2010 04:56|
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