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Receptor-Mediated Gene Delivery Approach Demonstrates the Role of 59-Proximal DNA Region in Conferring Phenobarbitone Responsiveness to CYP2B2 Gene in Rat Liver in Vivo

Mani, SA and Harish, S and Vathsala, PG and Rangarajan, PN and Padmanaban, G (2000) Receptor-Mediated Gene Delivery Approach Demonstrates the Role of 59-Proximal DNA Region in Conferring Phenobarbitone Responsiveness to CYP2B2 Gene in Rat Liver in Vivo. In: Biochemical and Biophysical Research Communications, 268 (03). pp. 734-739.

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Abstract

The phenobarbitone (PB) responsiveness of the 5'-proximal region of the CYP2B1/B2 gene was examined in detail with plasmid DNA constructs containing G-free cassette as reporter, using in vivo targeting of the same DNA constructs into rat liver as ffalactosylated-polylysine complexes. The contribution of the proximal region (-1 to -179 bp) and the positive element (-69 to -98 bp) identified earlier in this laboratory to PB responsiveness was assessed. The results obtained on PB treatment of rats subjected to receptor-mediated gene delivery to liver were conclusive and dramatic, with the control (saline-treated) rats manifesting very little expression of the reporter, reflecting the in vivo picture of CYP2B1/B2 gene expression. The positive element conferred PB responsiveness to homologous and heterologous promoters. Deletion of the positive element led to elimination of PB response. The entire -179 bp region was significantly more effective in responding to PB treatment than the region up to -98 bp, both containing one copy of the positive element. Thus, the positive element and its flanking sequences in the 5'-proximal region are involved in conferring PB responsiveness to the CYP2B1/B2 gene

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Nov 2009 05:31
Last Modified: 19 Sep 2010 05:00
URI: http://eprints.iisc.ernet.in/id/eprint/18192

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