Thanedar, Swapna and Dineshkumar, TK and Varshney, Umesh (2001) The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli. In: Journal of Bacteriology, 183 (24). pp. 7397-7402.
Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli (defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation- independent initiation of protein synthesis.
|Item Type:||Journal Article|
|Additional Information:||The copyright of this article belongs to American Society for Microbiology.|
|Keywords:||Formylmethionine transfer-rna;Protein synthesis;Streptococcus-faecalis;Recognition;Methionine;Stem|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||09 Sep 2004|
|Last Modified:||19 Sep 2010 04:16|
Actions (login required)