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Regulation of peri-attachment embryo development in the golden hamster: role of growth factors

Seshagiri, PB and Mishra, A and Ramesh, G and Rao, RP (2002) Regulation of peri-attachment embryo development in the golden hamster: role of growth factors. In: Journal of Reproductive Immunology, 53 (1-2). pp. 203-213.

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Abstract

The molecular regulation of mammalian peri-implantation development is complex and difficult to study in vivo. We successfully cultured hamster blastocysts through hatching and peri-attachment stages, using a chemically defined medium, HECM-2h. Using this system, we showed that a species-specific, embryonic cysteine-like protease is involved in blastocyst hatching and that the process is modulated by growth factors. In particular, heparin binding-epidermal growth factor (HB-EGF) or leukemia inhibitory factor (LIF) enhance blastocyst hatching, and the former also improves attachment and trophoblast outgrowth. We observed interesting changing patterns of expression of mRNA and/or immunoreactive protein for EGF, HB-EGF, LIF and transforming growth factor-beta (TGF-beta) in the embryo and/or endometrial tissue, during peri-implantation development. Together, it appears that hamster blastocyst hatching, attachment and trophoblast outgrowth are regulated by autocrine and/or paracrine growth factors, produced by the embryo-endometrial tissues. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 23 Nov 2009 08:20
Last Modified: 19 Sep 2010 05:24
URI: http://eprints.iisc.ernet.in/id/eprint/18641

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