Dam, Tarun K and Bachhawat, Kiran and Rani, Geetha P and Surolia, Avadhesha (1998) Garlic (Allium sativum) Lectins Bind to High Mannose Oligosaccharide Chains. In: Journal of Biological Chemistry, 273 (10). pp. 5528-5535.
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Two mannose-binding lectins, Allium sativum agglutinin (ASA) I (25 kDa) and ASAIII (48 kDa), from garlic bulbs have been purified by affinity chromatography followed by gel filtration. The subunit structures of these lectins are different, but they display similar sugar specificities. Both ASI and ASAIII are made up of 12.5- and 11.5-kDa subunits. In addition, a complex (136 kDa) comprising a polypeptide chain of 54 +/- 4 kDa and the subunits of ASAI and ASAIII elutes earlier than these lectins on gel filtration. The 54-kDa subunit is proven to be alliinase, which is known, to form a complex with garlic lectins. Constituent subunits of ASAI and ASAIII exhibit the same sequence at their amino termini. ASAI and ASAIII recognize monosaccharides in mannosyl configuration. The potencies of the ligands for ASAs increase in the following order: mannobiose (Man alpha 1-3Man) < mannotriose (Man alpha 1-6Man alpha 1-3Man) approximate to mannopentaose much less than man(9)-oligosaccharide. The addition of two GIcNAc residues at the reducing end of manotriose or mannopentaose enhances their potencies significantly, whereas substitution of both alpha 1-3- and alpha 1-6-mannosyl residues of mannotriose with GlcNAc at the nonreducing end increases their activity only marginally. The best manno-oligosaccharide ligand is Man(9)GlcNAc(2)Asn, which bears several alpha 1-2-linked mannose residues. Interaction with glycoproteins suggests that these lectins recognize internal mannose as well as bind to the core pentasaccharide of N-linked glycans even when it is sialylated. The strongest inhibitors are the high mannose-containing glycoproteins, which carry larger glycan chains. Indeed, invertase, which contains 85% of its mannose residues in species larger than Man(20)GlcNAc, exhibited the highest binding affinity. No other mannose- or mannose/glucose-binding lectin has been shown to display such a specificity.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Society for Biochemistry and Molecular Biology, Inc.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||22 Jul 2009 11:32|
|Last Modified:||19 Sep 2010 05:24|
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