Saini, Sangeeta and Singh, Harjinder and Bagchi, Biman (2006) Fluorescence resonance energy transfer (FRET) in chemistry and biology: Non-Forster distance dependence of the FRET rate. In: International Conference on Recent Developments in Metal Oxides and Related Materials Bangalore, INDIA, JAN 09-11, 2006, http://www.ias.ac.in/chemsci/Pdf-Jan2006/23.pdf.
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Fluorescence resonance energy transfer (FRET) is a popular tool to study equilibrium and dynamical properties of polymers and biopolymers in condensed phases and is now widely used in conjunction with single molecule spectroscopy. In the data analysis, one usually employs the Forster expression which predicts (1/R-6) distance dependence of the energy transfer rate. However, critical analysis shows that this expression can be of rather limited validity in many cases. We demonstrate this by explicitly considering a donor-acceptor system, polyfluorene (PF6)-tetraphenylporphyrin (TPP), where the size of both donor and acceptor is comparable to the distance separating them. In such cases, one may expect much weaker distance (as 1/R-2 or even weaker) dependence. We have also considered the case of energy transfer from a dye to a nanoparticle. Here we find 1/R-4 distance dependence at large separations, completely different from Forster. We also discuss recent application of FRET to study polymer conformational dynamics.
|Item Type:||Conference Paper|
|Additional Information:||Copyright of this article belogs to Indian Academy of Sciences.|
|Keywords:||Fluorescence resonance energy transfer (FRET);non-Förster regime;single molecule spectroscopy;donor–acceptor system.|
|Department/Centre:||Division of Chemical Sciences > Solid State & Structural Chemistry Unit|
|Date Deposited:||24 Mar 2009 06:46|
|Last Modified:||19 Sep 2010 05:25|
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