Vasanthakrishna, M and Rumpal, N and Varshney, U (1998) Organization and copy number of initiator tRNA genes in slow- and fast-growing mycobacteria. In: Journal of Biosciences, 23 (02). pp. 101-110.
Organization_and_copy_number.pdf - Published Version
We have previously reported the isolation an characterization of a functional initiator tRNA gene, metA, and a second initiator tRNA-like sequence, metB, from Mycobacterium tuberculosis. Here we describe the fine mapping of the initiator tRNA gene locus of the avirulent (H37Ra) and virulent (H37Rv) strains of M. tuberculosis. The genomic blot analyses show that the 1.7 kb (harbouring metB) and the 6.0 kb BamHI (harbouring metA) fragments are linked. Further, sequencing of a portion of the 6.0 kb fragment, in conjunction with the sequence of the 1.7 kb fragment confirmed the presence of an IS6110 element in the vicinity of metB. The IS element is flanked by inverted (28 bp, with 3 contiguous mismatches in the middle) and direct (3 bp) repeats considered to be the hallmarks of IS6110 integration sites. The organization of the initiator tRNA gene locus is identical in both the H37Ra and H37Rv strains and they carry a single copy of the functional initiator tRNA gene. Interestingly, the fast growing Mycobacterium smegmatis also bears a single initiator tRNA gene. This finding is significant in view of the qualitative differences in total tRNA pools and the copy number of rRNA genes in the fast-and slow-growing mycobacteria. Finally, we discuss hypotheses related to the origin of metB in M. tuberculosis.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian Academy of Sciences.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||29 Dec 2009 08:19|
|Last Modified:||19 Sep 2010 05:26|
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