ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Antigenic similarities among biotin-binding proteins

Subramanian, N and Subramanian, Sarada and Adiga, Radhakantha P (1996) Antigenic similarities among biotin-binding proteins. In: Biochemistry and Molecular Biology International, 40 (04). pp. 663-670.

[img] PDF
ANTIGENIC_SIMILARITIES.pdf - Published Version
Restricted to Registered users only

Download (537Kb) | Request a copy
Official URL: http://www3.interscience.wiley.com/cgi-bin/fulltex...

Abstract

Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [H-3]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press.
Keywords: Biotin-binders;antibodies;ligand-binding;inhibition.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited: 26 Feb 2009 07:18
Last Modified: 01 Mar 2012 07:13
URI: http://eprints.iisc.ernet.in/id/eprint/19080

Actions (login required)

View Item View Item