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3' non-templated 'A' addition by Taq DNA polymerase: An advantage in the construction of single and double mutants

Jagath-Reddy, J and Rao, NA and Savithri, HS (1996) 3' non-templated 'A' addition by Taq DNA polymerase: An advantage in the construction of single and double mutants. In: Current Science, 71 (09). pp. 710-712.

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Official URL: http://www.ias.ac.in/j_archive/currsci/71/9/710-71...

Abstract

The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3' non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferase

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Current Science Association
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 19 Feb 2009 09:38
Last Modified: 19 Sep 2010 05:26
URI: http://eprints.iisc.ernet.in/id/eprint/19134

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