Sharma, Sujata and Gopinathan, Karumathil P (1996) Role of TATATAA element in the regulation of tRNA(1)(Gly) gene expression in Bombyx mori is position dependent. In: Journal of Molecular Biology., 262 (04). pp. 396-406.
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Transcription of tRNA genes by RNA polymerase III is controlled by the internal conserved sequences within the coding region and the immediate upstream flanking sequences. A highly transcribed copy of glycyl tRNA gene tRNA1(Gly)-1 from Bombyx mori is down regulated by sequences located much farther upstream in the region -150 to -300 nucleotides (nt), with respect to the +1 nt of tRNA. The negative regulatory effect has been narrowed down to a sequence motif 'TATATAA', a perfect consensus recognised by the TATA binding protein, TBP. This sequence element, when brought closer to the transcription start point, on the other hand, exerts a positive effect by promoting transcription of the gene devoid of other cis regulatory elements. The identity of the nuclear protein interacting with this 'TATATAA' element to TBP has been established by antibody and mutagenesis studies. The 'TATATAA' element thus influences the transcription of tRNA genes positively or negatively in a position-dependent manner either by recruitment or sequestration of TBP from the transcription machinery.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press.|
|Keywords:||pol III transcription; silkworm;TATA-binding protein (TBP); transcriptional regulation;tRNA(1)(gly) genes|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||04 Dec 2009 09:27|
|Last Modified:||19 Sep 2010 05:28|
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