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Protection of rabbits against lapinized rinderpest virus with purified envelope glycoproteins of peste-des-petits-ruminants and rinderpest viruses

Devireddy, LR and Raghavan, R and Ramachandran, S and Subbarao, SM (1998) Protection of rabbits against lapinized rinderpest virus with purified envelope glycoproteins of peste-des-petits-ruminants and rinderpest viruses. In: Acta virologica, 42 (05). pp. 299-306.

Full text not available from this repository.
Official URL: http://www.sav.sk/journals/acta/acta598.htm#4

Abstract

Haemagglutinin (HA) and fusion (F) proteins of peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) were purified by immunoaffinity chromatography. The purified proteins were characterized by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Rabbit hyperimmune sera were raised against the purified HA and F proteins and assayed by enzyme-linked immunosorbent assay (ELISA), haemagglutination-inhibition (HAI) and virus neutralization (VN) tests. The immunized animals were challenged with a virulent lapinized (rabbit-adapted) strain of RPV: Both HA and F proteins of PPRV protected rabbits against a lethal challenge with lapinized RPV. As expected, RPV HA and F proteins also conferred a similar protection against the homologous challenge. The postchallenge antibody responses were of a true anamnestic type.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Slovak Academic Press.
Keywords: peste-des-petits-ruminants;rinderpest;vaccine;viruses; immunity.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 27 Nov 2009 05:45
Last Modified: 27 Nov 2009 05:45
URI: http://eprints.iisc.ernet.in/id/eprint/19537

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