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A soluble preparation from developing groundnut seeds (Arachis hypogaea) catalyzes de novo synthesis of long chain fatty acids

Sreenivas, A and Sastry, PS (1995) A soluble preparation from developing groundnut seeds (Arachis hypogaea) catalyzes de novo synthesis of long chain fatty acids. In: Indian Journal Of Biochemistry & Biophysics, 32 (4). pp. 213-217.

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Official URL: http://www.niscair.res.in/sciencecommunication/res...

Abstract

A 100,000 x g supernatant fraction prepared from developing groundnut seeds (30-35 days after flowering) catalyzed the synthesis of fatty acids from [l-14C]acetate at a rate of 120nmoles of acetate incorporated per hr per gram fresh weight of tissue. 90% of this incorporated label was associated with fatty acids. The major fatty acids formed were stearic- (77%) and palmitic acids (14%) with 4% of oleic acid. The fatty acid synthetase activity was stable when stored at 0-4 degrees C for at least fifteen days. It is concluded from these results that acetyl-coA carboxylase and all the enzymes of fatty acid synthetase from developing groundnut seeds are soluble.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Council Scientific Industrial Research
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 19 Feb 2010 07:04
Last Modified: 19 Feb 2010 07:04
URI: http://eprints.iisc.ernet.in/id/eprint/19628

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