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Antibodies to guanosine: Fractionation and specificities

Iswari, S and Jacob, TM (1984) Antibodies to guanosine: Fractionation and specificities. In: FEBS letters, 176 (1). pp. 43-48.

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Abstract

Bovine serum albumin conjugates of guanosine prepared by the periodate method was used as immunogen to elicit guanosine antibodies in rabbits. The specificities of the antibodies were studied by the inhibition of their binding to [3H]Gox-red, [32P]DNA and [3H]RNA by related non-radioactive compounds. A population of antibodies is specific to Gox-red with an average association constant of around 107M−1 at 4°C. There are a population of antibodies which bind to [32P]ssDNA and [3H]RNA specifically at guanosine residues. RNA binding antibodies were separated into two populations by affinity chromatography.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to Elsevier Science.
Keywords: Guanosine; Antibody binding.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 26 May 2009 05:30
Last Modified: 19 Sep 2010 05:32
URI: http://eprints.iisc.ernet.in/id/eprint/20391

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