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Crystallization and preliminary X-ray studies of snake gourd lectin: homology with type II ribosome-inactivating proteins

Manoj, N and Jeyaprakash, Arockia A and Pratap, JV and Komath, Sneha Sudha and Kenoth, Roopa and Swamy, Musti J and Vijayan, M (2001) Crystallization and preliminary X-ray studies of snake gourd lectin: homology with type II ribosome-inactivating proteins. In: Acta Crystallographica Section D, 57 . pp. 912-914.

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Abstract

The lectin from the seeds of snake gourd (Trichosanthes anguina) has been crystallized in two forms using the hanging-drop method. Both the forms are hexagonal, with the asymmetric unit containing one subunit consisting of two polypeptide chains linked through disulfide bridges. Intensity data from one of the forms were collected at room temperature as well as at low temperature to 3 A resolution. Molecular-replacement studies indicate that the lectin is homologous to type II ribosome-inactivating proteins. Partial refinement confirms this conclusion.

Item Type: Journal Article
Additional Information: The copyright belongs to the International Union of Crystallography.
Keywords: crystallization;x ray studies;lectin;ribosome;inactivating proteins;trichosanthes anguina;snake gourd
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 16 Sep 2004
Last Modified: 19 Sep 2010 04:16
URI: http://eprints.iisc.ernet.in/id/eprint/2044

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