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Catalysis and mechanism of malonyl transferase activity in type II fatty acid biosynthesis acyl carrier proteins

Misra, Ashish and Surolia, Namita and Surolia, Avadhesha (2009) Catalysis and mechanism of malonyl transferase activity in type II fatty acid biosynthesis acyl carrier proteins. In: Molecular BioSystems, 5 (6). pp. 651-659.

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Abstract

One of the unexplored, yet important aspects of the biology of acyl carrier proteins (ACPs) is the self-acylation and malonyl transferase activities dedicated to ACPs in polyketide synthesis. Our studies demonstrate the existence of malonyl transferase activity in ACPs involved in type II fatty acid biosynthesis from Plasmodium falciparum and Escherichia coli. We also show that the catalytic malonyl transferase activity is intrinsic to an individual ACP. Mutational analysis implicates an arginine/lysine in loop II and an arginine/glutamine in helix III as the catalytic residues for transferase function. The hydrogen bonding properties of these residues appears to be indispensable for the transferase reaction. Complementation of fabD(Ts) E. coli highlights the putative physiological role of this process. Our studies thus shed light on a key aspect of ACP biology and provide insights into the mechanism involved therein.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Royal Society of Chemistry.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 01 Jul 2009 11:30
Last Modified: 19 Sep 2010 05:34
URI: http://eprints.iisc.ernet.in/id/eprint/20728

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