Srivenugopal, KS and Adiga, PR (1980) Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings. In: Bochemical Journal, 190 (2). pp. 461-464.
fulltext.pdf - Published Version
Restricted to Registered users only
Download (648Kb) | Request a copy
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to the Biochemical Society.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||17 Jul 2009 06:55|
|Last Modified:||01 Mar 2012 06:37|
Actions (login required)