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Studies on the purification of banana polyphenoloxidase

Jayaraman, KS and Ramanuja, MN and Vijayaraghavan, PK and Vaidyanathan, CS (1987) Studies on the purification of banana polyphenoloxidase. In: Food Chemistry, 24 (3). pp. 203-217.

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Abstract

Studies on the extractability of polyphenoloxidase (PPO) from the pulp of five banana cultivars revealed a varietal difference in the nature of binding of the PPO in the cell, with the enzyme being entirely in the soluble fraction in one and partly associated with the cell wall in others, necessitating use of a detergent to release it from the latter. Partial purification by acetone precipitation and chromatography using a DEAE-cellulose column yielded two major fractions DE-I and DE-II with purifications of 4- and 16·3-fold and activity recoveries of 38·2 and 43·3% respectively. Further gel filtration of the two fractions on a Sephadex G-100 column improved the purifications to 44- and 50-fold respectively with full activity recovery. Polyacrylamide gel electrophoretic studies showed the two fractions to be composed of isoenzymes differing in pattern. The purified enzyme showed maximum absorption at 275 nm.

Item Type: Journal Article
Additional Information: Copy right of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 12 Jan 2010 06:58
Last Modified: 19 Sep 2010 05:36
URI: http://eprints.iisc.ernet.in/id/eprint/21264

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