ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Linalyl Acetate Is Metabolized by Pseudomonas incognita with the Acetoxy Group Intact

Renganathan, V and Madyastha, KM (1983) Linalyl Acetate Is Metabolized by Pseudomonas incognita with the Acetoxy Group Intact. In: Applied and Environmental Microbiology, 45 (1). pp. 6-15.

[img] PDF
6.pdf - Published Version
Restricted to Registered users only

Download (1508Kb) | Request a copy
Official URL: http://aem.asm.org/cgi/content/abstract/45/1/6

Abstract

Metabolism of linalyl acetate by Pseudomonas incognita isolated by enrichment culture on the acyclic monoterpene alcohol linalool was studied. Biodegradation of linalyl acetate by this strain resulted in the formation of linalool, linalool- 8-carboxylic acid, oleuropeic acid, and A5-4-acetoxy-4-methyl hexenoic acid. Cells adapted to linalyl acetate metabolized linalyl acetate-8-aldehyde to linalool- 8-carboxylic acid, linalyl acetate-8-carboxylic acid, A5-4-acetoxy-4-methyl hexenoic acid, and geraniol-8-carboxylic acid. Resting cell suspensions previously grown with linalyl acetate oxidized linalyl acetate-8-aldehyde to linalyl acetate-8- carboxylic acid, A5-4-acetoxy-4-methyl hexenoic acid, and pyruvic acid. The crude cell-free extract (10,000 g of supernatant), obtained from the sonicate of linalyl acetate-grown cells, was shown to contain enzyme systems responsible for the formation of linalyl acetate-8-carboxylic acid and linalool-8-carboxylic acid from linalyl acetate. The same supernatant contained NAD-linked alcohol and aldehyde dehydrogenases involved in the formation of linalyl acetate-8-aldehyde and linalyl acetate-8-carboxylic acid, respectively. On the basis of various metabolites isolated from the culture medium, resting cell experiments, growth and manometric studies carried out with the isolated metabolites as well as related synthetic analogs, and the preliminary enzymatic studies performed with the cellfree extract, a probable pathway for the microbial degradation of linalyl acetate with the acetoxy group intact is suggested.

Item Type: Journal Article
Additional Information: Copyright of this article belongsto American Society for Microbiology.
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Date Deposited: 04 Sep 2009 03:16
Last Modified: 19 Sep 2010 05:37
URI: http://eprints.iisc.ernet.in/id/eprint/21500

Actions (login required)

View Item View Item