Ramachandraiah, Gosu and Chandra, Nagasuma R and Surolia, A and Vijayan, M (2003) Computational analysis of multivalency in lectins: structures of garlic lectin± oligosaccharide complexes and their aggregates. In: Glycobiology, 13 (11). pp. 765-775.
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Multivalency in lectins is a phenomenon that has been discussed at considerable length. The structural basis for the role of multivalency in garlic lectin has been investigated here through computational studies. Biochemical studies have shown that the binding affinity of garlic lectin for high mannose oligosaccharides is orders of magnitude greater than that for mannose. Modeling and energy calculations clearly indicate that such increase in affinity cannot be accounted for by binding of these oligosaccharides at any of the six sites of a garlic lectin dimer. These studies also indicate that a given oligosaccharide cannot bind simultaneously to more than one binding site on a lectin dimer. The possibility of a given oligosaccharide simultaneously binding to and hence linking two or more lectin molecules was therefore explored. This study showed that trimannosides and higher oligomers can cross-link lectin dimers, amplifying the protein±oligosaccharide interactions severalfold, thus explaining the role of multivalency in enhancing affinity. A comprehensive exploration of all possible cross-links posed a formidable computational problem. Even a partial exploration involving a carefully chosen region of the conformational space clearly showed that a given dimer pair can be cross-linked not only by a single oligosaccharide molecule but also simultaneously by two oligosaccharides. The number of such possible double crosslinks, including those forming interesting tetrameric structures, generally increases with the size of the oligosaccharide,correlating with the biochemical data. In addition to their immediate relevance to garlic lectin, these studies are of general interest in relation to lectin±oligosaccharide interactions.
|Item Type:||Journal Article|
|Additional Information:||The copyright belongs to Oxford University Press.|
|Keywords:||affinity enhancement;cross-linking;multiple binding sites;oligosaccharide binding|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||25 Oct 2004|
|Last Modified:||19 Sep 2010 04:16|
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