Varalakshmi, K and Savithri, HS and Rao, NA (1986) Identification of amino acid residues essential for enzyme activity of sheep liver 5,10-methylenetetrahydrofolate reductase. In: Biochemical Journal, 236 (1). 295 -298.
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Abstract
Sheep liver 5,10-methylenetetrahydrofolate reductase was subjected to specific chemical modification with phenylglyoxal, diethyl pyrocarbonate and N-bromosuccinimide. The second-order rate constants for inactivation were calculated to be 54 M-1 X min-1, 103 M-1 X min-1 and 154 M-1 X min-1 respectively. This inactivation could be prevented by incubation with substrates or products, suggesting that the residues modified, namely arginine, histidine and tryptophan, are essential for enzyme activity.
| Item Type: | Journal Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Portland Press. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 12 Aug 2009 11:16 |
| Last Modified: | 19 Sep 2010 05:39 |
| URI: | http://eprints.iisc.ernet.in/id/eprint/21902 |
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