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Spore and crystal formation in Bacillus thuringiensis var thuringiensis during growth in cystine and cysteine.

Rajalakshmi, S and Shethna, YI (1980) Spore and crystal formation in Bacillus thuringiensis var thuringiensis during growth in cystine and cysteine. In: Journal Of Biosciences, 2 (4). pp. 321-328.

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Abstract

The effect of the addition of different concentratons of cystine and cysteine on sporulation and parasporal crystal formation in Bacillus thuringiensis var. thuringiensis was studied. The effect was well pronounced when the systine/cysteine additions were made after the stationary phase. Heat stable spores and crystals were formed when the culture was provided with a low concentration of cystine/cysteine (0.05 per cent w/v). At a moderate concentration of cystine or cysteine (0.15%), only heat labile spores were formed without the production of the crystal. When the cystine/cysteine concentration was high (0.25%), spore and crystal formation were completely inhibited. Partial reversal of inhibition of sporulation was brought about by sodium sulphate or zinc sulphate and lead, copper, cadmium or cobalt acetate at 0.2 mM or at 0.2% of sodium or potassium pyruvate, citrate, isaconitate, oxalosuccinate, ∝ -keto-glutarate, succinate, fumarate, malate, or oxalacetate. Glutamate (0.2%) overcame the inhibitory effect of cystine/cysteine completely. The structural changes observed using phase contrast microscopy were dependent upon the concentration of cystine/cysteine.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian Academy Sciences.
Keywords: Bacillus thuringiensis var. thuringiensis. cystine, cysteine, spore, crystal, reversal of inhibition.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 25 Jan 2010 07:37
Last Modified: 27 Dec 2010 09:33
URI: http://eprints.iisc.ernet.in/id/eprint/22160

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