Vijayasarathy, S and Balaram, P (1982) Regional differentiation in bull sperm plasma-membranes. In: Biochemical and Biophysical Research Communications, 108 (2). pp. 760-769.
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Abstract
Bull sperm heads and tails have been separated by proteolytic digestion (trypsin) and plasma membranes have been isolated, using discontinuous sucrose density gradient centrifugation. Plasma membrane bound Ca2+-ATPase is shown to be associated mostly with the tail membranes. Pyrene excimer fluorescence and diphenylhexatriene fluorescence polarization experiments indicate a more fluid lipid phase in the tail region. Differences in surface charge distribution have been found, using 1-anilinonaphthalene-8-sulfonate and Tb3+ as fluorescent probes.
| Item Type: | Journal Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 30 Dec 2009 11:28 |
| Last Modified: | 19 Sep 2010 05:41 |
| URI: | http://eprints.iisc.ernet.in/id/eprint/22404 |
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