Deobagkar, DN and Shankar, V and Deobagkar, DD (1986) Separation of 5-methylcytosine-rich DNA using immobilized antibody. In: Enzyme and Microbial Technology, 8 (2). pp. 97-100.
http___www.sciencedirect.com_science__ob=MImg&_imagekey=B6TG1-47GMHN9-K1-1&_cdi=5241&_user=512776&_orig=search&_coverDate=02%2F28%2F1986&_sk=999919997&view=c&wchp=dGLzVtz-zSkzV&md5=a8d4981f9.pdf - Published Version
Restricted to Registered users only
Download (645Kb) | Request a copy
Anti-(5-methylcytosine) antibodies were immobilized on glutaraldehyde-activated Indion 48-R, a polystyrene resin with amino groups. Immobilized antibody was very stable and could be used several times without any apparent change in the initial binding capacity of the antibody-matrix. Fractions of total DNA from various animal and plant sources were retained on this column and could be eluted quantitatively with 1.0 m NaCl. The bound fraction was further characterized for its 5-methylcytosine content by restriction enzyme digestion patterns.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||27 Jan 2010 07:03|
|Last Modified:||19 Sep 2010 05:43|
Actions (login required)