ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Separation of 5-methylcytosine-rich DNA using immobilized antibody

Deobagkar, DN and Shankar, V and Deobagkar, DD (1986) Separation of 5-methylcytosine-rich DNA using immobilized antibody. In: Enzyme and Microbial Technology, 8 (2). pp. 97-100.

[img] PDF
http___www.sciencedirect.com_science__ob=MImg&_imagekey=B6TG1-47GMHN9-K1-1&_cdi=5241&_user=512776&_orig=search&_coverDate=02%2F28%2F1986&_sk=999919997&view=c&wchp=dGLzVtz-zSkzV&md5=a8d4981f9.pdf - Published Version
Restricted to Registered users only

Download (645Kb) | Request a copy
Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...

Abstract

Anti-(5-methylcytosine) antibodies were immobilized on glutaraldehyde-activated Indion 48-R, a polystyrene resin with amino groups. Immobilized antibody was very stable and could be used several times without any apparent change in the initial binding capacity of the antibody-matrix. Fractions of total DNA from various animal and plant sources were retained on this column and could be eluted quantitatively with 1.0 m NaCl. The bound fraction was further characterized for its 5-methylcytosine content by restriction enzyme digestion patterns.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 27 Jan 2010 07:03
Last Modified: 19 Sep 2010 05:43
URI: http://eprints.iisc.ernet.in/id/eprint/22725

Actions (login required)

View Item View Item