Rao, S Nageswara and Cherayil, Joseph D (1982) Evidence for Non-coordinated synthesis of 5 S RNA in the thermophilic fungus, Thermomyces lanuginosus. In: Journal of Biosciences, 4 (2). pp. 239-244.
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Analysis of ribosomes and the post ribosomal supernatant fraction of actively growing cells ofThermomyces lanuginosus showed the presence of free 5 S RNA in the supernatant fraction. This 5 S RNA was identical to the ribosomal 5 S RNA in its electrophoretic mobility on 10% Polyacrylamide gel and in its base composition. 5 S RNA from both the sources gave evidence for the presence of diphosphate at the 5’ end. Most of the 5 S RNA that appeared in the cytoplasm was that transported from the nucleus during the isolation. This could be prevented by the use of a hexylene glycol-HEPES buffer.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Springer.|
|Keywords:||5 S RNA - thermophilic fungus - ribosomal RNA - non-coordinated synthesis of PNA|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||24 Dec 2009 11:13|
|Last Modified:||19 Sep 2010 05:43|
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