Subramanian, Vasantha and Rao, PV Subra (1980) In vitro culture of the allergenic weed Parthenium hysterophorus L. In: Plant Science Letters, 17 (3). pp. 269-277.
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Excised stem, leaf segments and whole flower of the allergenic weed P. hysterophorus were cultured on Murashighe and Skoog's basal medium supplemented with hormones. Shoot buds readily formed in the stem callus cultured on MS Medium supplemented with IAA and BAP or Kinetin. The leaf callus formed roots alone in a wide variety of media. Suspension cultures were initiated from the leaf and stem callus. The leaf callus elicited a positive patch test response for delayed hypersensitivity in 4 patients suffering from Parthenium dermatitis, thus indicating its ability to synthesise the allergenic principle(s).
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier.|
|Keywords:||Bap, 6-benzylamino purine; CM, coconut milk; 2,4-D, 2,4-dichlorophenoxyacetic acid; GI, growth index; IAA, 3-indoleacetic acid; Kn, Kinetin; MS; Murashige; Skoog's basal nutrient medium; NAA, α-naphthalene acetic acid; Z, zeatin|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||02 Feb 2010 10:13|
|Last Modified:||19 Sep 2010 05:44|
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