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Number and proportion of selenocucleosides in the transfer RNA of escherichia-coli

Rao, YS Prasada and Cherayil, Joseph D (1974) Number and proportion of selenocucleosides in the transfer RNA of escherichia-coli. In: Life Sciences, 14 (10). pp. 2051-2059.

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Abstract

tRNA isolated from escherichia-coli grown in a medium containing [75Se] sodium selenosulfate was converted to nucleosides and analysed for selenonucleosides on a phosphocellulose column. Upon chromatography of the nucleosides on phosphocellulose column, the radioactivity resolved into three peaks. The first peak consisted of free selenium and traces of undigested nucleotides. The second peak was identified as 4-selenouridine by co-chromatographing with an authentic sample of 4-selenouridine. The identity of the third peak was not established. The second and third peaks represented 93% and 7% of the selenium present in nucleosides respectively.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 19 Dec 2009 07:43
Last Modified: 19 Sep 2010 05:45
URI: http://eprints.iisc.ernet.in/id/eprint/23296

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