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Anthranilate Hydroxylase from Aspergillus niger: Evidence for the Participation of Iron in the Double Hydroxylation Reaction

Kumar, Prema R and Sreeleela, NS and Rao, Subba PV and Vaidyanathan, CS (1973) Anthranilate Hydroxylase from Aspergillus niger: Evidence for the Participation of Iron in the Double Hydroxylation Reaction. In: Journal of Bacteriology, 113 (3). 1213-1216.

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Official URL: http://jb.asm.org/cgi/content/abstract/113/3/1213

Abstract

Evidence was obtained for the participation of iron in the double hydroxylation reaction catalyzed by anthranilate hydroxylase from Aspergillus niger (UBC 814). Omission of iron from the growth medium gave inactive preparations of anthranilate hydroxylase which could be reactivated by incubating the enzyme preparations with ferric citrate. The enzyme was susceptible to inhibition by metal chelating agents. The Ki for o-phenanthroline, which inhibited the enzyme activity non-competitively with respect to anthranilate, was calculated to be 0.9 mM. The inhibition by o-phenanthroline was counteracted by ferric complexes such as ferric-ethylenediaminetetraacetic acid and ferric citrate. Anthranilate afforded protection against inhibition by o-phenanthroline.

Item Type: Journal Article
Additional Information: Copy right of this article belongs to American Society for Microbiology
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 30 Sep 2009 09:05
Last Modified: 19 Sep 2010 05:46
URI: http://eprints.iisc.ernet.in/id/eprint/23462

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