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Allosteric serine hydroxymethyltransferase from monkey liver: temperature induced conformational transitions

Ramesh, Kashi S and Ananthanarayanan, VS and Rao, Appaji N (1981) Allosteric serine hydroxymethyltransferase from monkey liver: temperature induced conformational transitions. In: Journal Of Biosciences, 3 (2). pp. 179-190.

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Abstract

The homogeneous serine hydroxymethyltransferase from monkey liver was optimally activate at 60°C and the Arrhenius plot for the enzyme was nonlinear with a break at 15°C. The monkey liver enzyme showed high thermal stability of 62°C, as monitored by circular dichroism at 222 nm, absorbance at 280 nm and enzyme activity. The enzyme exhibited a sharp co-operative thermal transition in the range of 50°-70° (Tm= 65°C), as monitored by circular dichroism. L-Serine protected the enzyme against both thermal inactivation and thermal disruption of the secondary structure. The homotropic interactions of tetrahydrofolate with the enzyme was abolished at high temperatures (at 70°C, the Hill coefficient value was 1.0). A plot of h values vs. assay temperature of tetrahydrofolate saturation experiments, showed the presence of an intermediate conformer with an h value of 1.7 in the temperature range of 45°-60°C. Inclusion of a heat denaturation step in the scheme employed for the purification of serine hydroxymethyltransferase resulted in the loss of cooperative interactions with tetrahydrofolate. The temperature effects on the serine hydroxylmethyltransferase, reported for the first time, lead to a better understanding of the heat induced alterations in conformation and activity for this oligomeric protein.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian Academy Sciences
Keywords: Serine hydroxymethyltransferase;circular dichroism;thermal stability;conformational transitions.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 29 Sep 2009 06:21
Last Modified: 03 Aug 2011 10:37
URI: http://eprints.iisc.ernet.in/id/eprint/23579

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