Chandrashekaran, Siddamadappa and Babu, Padmanabhan and Nagaraja, Valakunja (1999) Purification and Characterization of Restriction Endonuclease Basl from Bacillus species. In: Journal Biochemistry, Molecular Biolology & Biophysics, 3 . pp. 225-229.
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After screening a variety of bacteria from soil for the DNA cleavage activity, a type II restriction endonuclease, Basl was isolated and characterized from a Bacillus species. Using the purified enzyme the recognition sequence was determined. The enzyme recognizes 5'- CCANNNN NTGG-3' sequence and cleaves as indicated. The enzyme functions efficiently at low ionic strength, narrow pH range and at the optimum temperature of 30°C.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Gordon and Breach Publishing Group.|
|Keywords:||Restriction endonuclease;Bacillus species;lsoschizomer|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||08 Dec 2004|
|Last Modified:||19 Sep 2010 04:17|
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