Sharma, Ram Vir and Mathur, Satya N and Dmitrovskii, AA and Das, Rathindra C and Ganguly, Jagannath (1977) Studies on the metabolism of Î²-carotene and apo-Î²-carotenoids in rats and chickens. In: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 486 (1). pp. 183-194.
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1. (1) The relative abilities of the various cell fractions of rat and chicken liver to oxidize and reduce retinal and 8'- and 12'-apo-Î²-carotenal were investigated and it has been shown that, while retinal is exclusively oxidized by the soluble fraction, the apocarotenals are mostly oxidized by the particulate fractions of the homogenate. 2. (2) Addition of NAD+ or NADP+ markedly activated the oxidation of the apocarotenals, but not of retinal by the particulate fractions. 3. (3) Considerable amounts of retinal and 8'-, 10'- and 12'-apo-Î²-carotenal were isolated from the intestine of chickens fed Î²-carotene and these apocarotenoids were conclusively identified. 4. (4) Significant amounts of 8'-, 10'- and 12'-apo-Î²-carotenoic acids were isolated from the intestine of rats given 8'-apo-Î²-carotenal and these apocarotenoic acids were also conclusively identified. 5. (5) In the light of these observations it is suggested that during conversion to vitamin A, the Î²-carotene molecule is simultaneously attacked by the dioxygenase at several double bonds, the primary attack being at the central double bond and a tentative scheme for the mechanism of conversion is proposed.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to Elsevier Science.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||21 Jan 2010 05:33|
|Last Modified:||19 Sep 2010 05:50|
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