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Unusual structural stability and ligand induced alterations in oligomerization of a galectin

Surolia, Avadhesha and Swaminathan, Chittoor P and Ramkumar, Radha and Podder, Sunil K (1997) Unusual structural stability and ligand induced alterations in oligomerization of a galectin. In: FEBS Letters, 409 (3). pp. 417-420.

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Abstract

Abstract L-14, a 14-kDa S-type lectin shows the jelly roll tertiary structural fold akin to legume lectins yet, unlike them, it does not dissociate on thermal unfolding. In the absence of ligand L-14 displays denaturation transitions corresponding to tetrameric and octameric entities. The presence of complementary ligand reduces the association of L-14, which is in stark contrast with legume lectins where no alterations in quaternary structures are brought about by saccharides. From the magnitude of the increase in denaturation temperature induced by disaccharides the binding constants calculated from differential scanning calorimetry are comparable with those extrapolated from titration calorimetry indicating that L-14 interacts with ligands essentially in the folded state.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Biochemistry
Date Deposited: 02 Feb 2010 08:03
Last Modified: 19 Sep 2010 05:51
URI: http://eprints.iisc.ernet.in/id/eprint/24588

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