Shetty, G and Krishnamurthy, H and Krishnamurthy, HN and Ramachandra, SG and Moudgal, NR (1997) Use of norethisterone and estradiol in mini doses as a contraceptive in the male : Efficacy studies in the adult male bonnet monkey (Macaca radiata). In: Contraception, 56 (4). pp. 257-265.
8.pdf - Published Version
Restricted to Registered users only
Download (979Kb) | Request a copy
Administration of norethisterone (NET) or NET + estradiol benzoate using an Alzet minipump or as once-a-month intramuscular injection of their depot forms, NET-enanthate (NET-EN) and estradiol valerate (E-val), resulted in azoospermia in all monkeys (n = 13) within 60 to 150 days of treatment. Although addition of depot form of testosterone (T, 20 mg/month) to the regimen restored the behavioral response typical of a normal male, it did not reverse the azoospermic state. Serum T (heightened nocturnal) levels were significantly reduced (> 85%, p < 0.001) in all the treated groups. Evidence for blockade in spermatogenesis following treatment was obtained by DNA flow cytometry. Following withdrawal of treatment, the T level was restored to normalcy within 15 days but 120 days more were required for the animals to exhibit normal sperm counts. In conclusion, the efficacy of once-a-month injection of relatively low doses of NET-EN + E-Val to bring about azoospermia in monkeys, in a relatively short time, has been demonstrated. As the results are uniform and reproducible, it appears desirable that this steroid regimen be tested in man for its contraceptive efficacy.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Keywords:||Author Keywords:azoospermia;progestins;spermatogenesis;male infertility;monkey.|
|Department/Centre:||Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)|
|Date Deposited:||22 Jan 2010 07:12|
|Last Modified:||19 Sep 2010 05:51|
Actions (login required)