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Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus

Purushothaman, SS and Park, HS and Cleary, PP (2004) Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus. In: Indian Journal of Medical Research, 119 . pp. 44-47.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/15232161


Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1DeltaAB (M1(-)) mutant was constructed, with thermosensitive pGhost vector. This isogenic M1(-) mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1(-) strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1(-) streptococci into HEp-2 cells. The M1(-) strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.

Item Type: Journal Article
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Additional Information: Copyright of this article belongs to Indian council of medical research.
Keywords: C5a peptidase;fibronectin;group A streptococcus;invasion;M1 strain.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 22 Jan 2010 05:21
Last Modified: 19 Sep 2010 05:51
URI: http://eprints.iisc.ernet.in/id/eprint/24746

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