Purushothaman, SS and Park, HS and Cleary, PP (2004) Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus. In: Indian Journal of Medical Research, 119 . pp. 44-47.
7.pdf - Published Version
Restricted to Registered users only
Download (56Kb) | Request a copy
Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1DeltaAB (M1(-)) mutant was constructed, with thermosensitive pGhost vector. This isogenic M1(-) mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1(-) strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1(-) streptococci into HEp-2 cells. The M1(-) strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Indian council of medical research.|
|Keywords:||C5a peptidase;fibronectin;group A streptococcus;invasion;M1 strain.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||22 Jan 2010 05:21|
|Last Modified:||19 Sep 2010 05:51|
Actions (login required)