Inhibition of replication of rinderpest virus by 5-fluorouracil

Ghosh, Arundhati and Nayak, Rabindranath and Shaila, MS (1996) Inhibition of replication of rinderpest virus by 5-fluorouracil. In: Antiviral Research, 31 (1-2). pp. 35-44.

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Abstract

5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%, at a concentration of 1 μg/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene—glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Rinderpest virus;5-Fluorouracil;Virus transcription; Antiviral drug.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	21 Jan 2010 05:32
Last Modified:	19 Sep 2010 05:51
URI:	http://eprints.iisc.ernet.in/id/eprint/24770

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